OCCURRENCE AND SENSITIVITY TO ANTIBIOTICS OF BACTERIA FOUND IN GILLS, BUCCAL CAVITY AND SKIN OF Hemichromis fasciatus, Brycinus macrolepidotus AND Hydrocynus forskalii FROM OGUN RIVER, ABEOKUTA, OGUN STATE
The morphometrics of Hemichromis fasciatus, Brycinus macrolepidotus and Hydrocynus forskalii from Ogun River were determined. The culture, isolation, and characterization ofÂ bacteria species, their sensitivity to antibiotics were carried out. There were significant differences (P>0.05) in the weight, standard length, head length, gill length and buccal depth. The highest mean body weight 113.85 Â± 9.38 g was observed in B. macrolepidotus while the lowest mean weight of 47.20 Â± 6.3 g was observed in H. forskalii. Ten (10) bacteria species were isolated from the gill, buccal cavity and skin. These include the Gram positive (Staphylococcus epidermidis, Streptococcus faecium, MicrococcusÂ letus) and the Gram negative bacteria (Enterobacter aerogenes, Pseudomonas aerogenes, Klebseilla aerogenes, AerobacterÂ aerogenes, Proteus vulgaris, Bacillus licheniform and Salmonella sp). The number of colony forming units per milligram varied from 3.1Ã—104 to 3.8Ã—105. The highest mean percentage bacteria occurrence of 5.4 Â± 4.4at (P> 0.05) was recorded from the gills of H. fasciatus while the lowest value of 4.6 Â± 3.8was recorded in the gill of H. forskalii S. epidermidis hadÂ the widest inhibition zones of 10.2 mm and 10.5 mm respectively of sensitivity to Sparfloxcin and Caflazidime while P. vulgaris had the thinnest inhibition zone 2.1 mm of resistance to Caflazidime. From the foregoing results, this study confirmed that the bacterial organisms isolated from the fish were pathogenic and of public health importance. Caflazidime and Oflaxacin were the best antibiotic substances with the highest inhibition zones of 10.5 mm and 10.0 mm for the Gram positive bacteria (S. epidermidis) and the Gram negative bacteria (E. aerogenes) respectively.
Akinyemi, A.A. 2001. Studies on Micro-organisms in Smoke-cured Fish Sold in Open Markets in Abeokuta, Nigeria: A desertification submitted to the Post-graduate School, University of Agriculture, Abeokuta, Pp. 116.
Ashokkumar, P. 2008. Bacterial Resistance to Antimicrobial Agents and Microbiological Quality among Escherichia coli isolated from Dry Fishes in South East Coast of India: Rumanian Society of Biological Sciences, 13(6): 3084-3089.
Ayansanwo, A. 1999. Demographic and Socio-economic profiles of artisanal fishers in Ogun state, Pp. 28
Azipour, K. 2009. Biochemical characterization of Lactic acid Bacterial isolated from Rainbow Trout (Oneorhynchus mylass) West of Azarbaiju, Iran. Research Journals of Biological Sciences 4(3): 324-326.
Ballarin, J.D., Hatton, J.P. 1979. Tilapia, A guide to their biology and culture in Africa. University of Stirling, Scotland Pp 174.
Bisen, P.S., Verma, K. 1994. Hand book of Microbiology. Published by Satish Kamar Sain for C B S Distributors. New Delhi, India.
FDF 2007. Fisheries Statistics 4th Edition.
Ita, E.O., Sado, E.K. 1985. A Summary of the Inventory Survey of Nigeria Inland Waters and Preliminary Estimates of their Yield Potentials: Fisheries Society of Nigeria, Conference Proceedings Pp. 22-2
Jara Z., Chodyniecki, A. 1999. Ichtiopathology - AR wroctaw (in Polish) Jay, J.M (1986): Modern Food Microbiology, Van Nastrand Reinhold New York, pp. 227.
Lewis, D.H., Plumb, J.A 1979. Principal proposal for Farm Raised Catfish. Southern
Cooperative Series: Pp. 18
Sarig, S. 1976. Fish diseases and their control in aquaculture, in Advances in Aquaculture. In: Pillay, T.V.R and Dill, Win. A. (Eds.): FAO `Technical Conference on Aquaculture. Japan, Pp. 190-197.
Sowunmi, A.A., Okunubi, M.A., Efuntoye, M.O. 2008. Occurrence of bacteria in gills and buccal cavity of Clarias gariepinus (Burchell, 1822) and Tilapia zilli (Gervais) from Lekki lagoon, South West Nigeria. World Journal of Biological Research, 001:1
Wei, L.S., Najiah, M., Faizah, S., Nendy, W. 2008. Surveillance of bacteria species in diseased freshwater ornamental fish: World Applied Science Journal, Pp. 402.