Keywords: Agar, Antibacterial activity, E. coli, Honey, Staphylococcus aureus


Honey has been used for various purposes including treatment of diseases. This study was carried out to determine antibacterial activities of honey samples obtained from different locations in the tropical Rainforest Belt of Nigeria (Abeokuta, Aiyetoro, Ajebo, and Saki) against reference bacterial isolates: Escherichia coli (E.coli) (ATCC 25922) and Staphylococcus aureus (ATCC 25923), using agar well diffusion technique. Staphylococcus aureus showed sensitivity to all honey samples used with the zone of inhibition ranging between 1.9 and 2.6mm. Escherichia coli was resistant to all concentrations of honey samples used. However, the present study did not explore the possible causative agent(s) involved in the antibacterial activity of the honey used.



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