CLINICAL MANIFESTATIONS IN RABBITS EXPERIMENTALLY INFECTED WITH ESCHERICHIA COLI O157:H7 ISOLATES OF DIFFERENT VIRULENCE GENE PROFILES

  • O. E. OJO Department of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, Federal University of Agriculture, Abeokuta, Abeokuta. Postcode 110001. Nigeria
  • A. T.P. AJUWAPE Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria
  • O. L. AJAYI Department of Veterinary Pathology, College of Veterinary Medicine, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria
  • E. B. OTESILE Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria
  • A. I. ADETOSOYE Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria
Keywords: Clinical manifestation E. coli O157:H7, experimental infection, rabbits, virulence, gene profile

Abstract

Escherichia coli O157:H7 is a major cause of zoonotic food-borne infections transmissible from asymptomatic animals to humans following consumption of contaminated foods. Pathogenicity of E. coli O157:H7 is attributed to possession of virulence genes such as eaeA and stx responsible for intimate adhesion to enterocytes and production of cytolethal shiga toxins. The pathogenic potentials of five E. coli O157:H7 isolates of different virulence gene profiles recovered from the faeces of slaughter cattle was compared in rabbit model. Five groups (A-E) of five rabbits were each inoculated orally with 5 x 109 colony forming units of an E. coli O157:H7 isolate possessing one of the virulence gene profiles: stx1 / stx2 / eaeA / hlyA (group A), stx1 /stx2 (B), stx1 (C), stx2 (D), and eaeA / hlyA (E). Group F (control) received sterile broth. The mean onset and duration of clinical manifestations varied significantly among the experimental groups being earliest and shortest in group infected with E. coli O157:H7 possessing stx1 / stx2 /eaeA / hlyA. Infected rabbits showed clinical signs including dullness, profuse non-bloody diarrhoea, weakness, anorexia and epistaxis starting from two days post infection (p.i.). Epistaxis was observed only in rabbits inoculated with isolates that possessed stx2 either alone or in combination with other virulence genes. Mortality of 100% was recorded in isolates with stx1 /stx2 /eaeA / hlyA, stx1 / stx2 and stxand 60% with stx1 and eaeA / hlyA.  Test organisms were detected in the faeces of inoculated animals as from two days p.i. and persisted in survivors for 19 to 30 days p.i. This study showed that E. coli O157:H7 isolates from cattle produced fatal illness in experimental rabbits and that virulence gene profile significantly influenced the onset, duration and severity of clinical manifestation of infection in the experimental animals.

 

 

 

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Published
2014-02-04
Section
Articles