MICRO-ORGANISMS ASSOCIATED WITH SMOKED PRAWN (Macrobranchium spp.) IN SELECTED MARKET LOCATIONS IN ABEOKUTA METROPOLIS OF OGUN STATE, NIGERIA
Keywords:Bacteria, fungi, microbial load, smoked prawn, spoilage.
This study is aimed at isolating and characterizing micro-organisms of Macrobranchium spp. gotten from markets in Abeokuta. Twelve different samples of whole smoked prawns (Macrobranchium spp.) were purchased from two different locations each in six major markets (Itoku, Omida, Iberekodo, Lafenwa, Panseke and Olomore) within the Abeokuta metropolis in Ogun State. Their microbial load was analyzed using Mac-Conkey agar (MA), Deoxycholate citrate agar (DCA), Nutrient agar (NA), and Mannitol salt agar (MSA) for bacteria isolation while Potato Dextrose agar (PDA) was used to isolate the fungi in the microbiology laboratory of the department of Microbiology, Federal University of Agriculture, Abeokuta. Staphylococcus aureus and Citrobacter spp (22.22% each) dominated the samples while the fungal specie that occurred most frequently in the samples was Aspergillus niger (31.03%). The total bacterial counts for all the samples ranged from 9x102 to 1.0x103 cfu/g and fungal count were between 21%-90% in terms of frequency of occurrence. These microorganisms cause food spoilage and poisoning and the occurrence of such microorganisms may be as a result unhygienic handling of prawns during processing as some of the microorganisms may be post-harvest contaminants. Adequate cooking could help in reducing microorganism of smoked prawn.
Chinivasagam, H.N., Bermner, H.A., Thrower, S.J., Nottingham, S.M. 1996.
Spoilage pattern of 5 species of Australian Prawn; Journal of Af. Food Prod tech, 5(1):
Daramola, J.A., Fasakin, E.A., Adeparusi, E.O. 2007. Changes in Physicochemical and Sensory Characteristics of Smoke-Dried Fish Species Stored at Ambient Temperature. African Journal of Food Agriculture Nutrition and Development, 7: 6
Edema, M.O., Agbon, A.O. 2010. Significance of fungi associated with smoke cured Etmalosa fimbriata and Clarias gariepinus. Journal of Food Processing and Preservation.
Fawole, M.O., Oso, B.A. 2001. Laboratory manual in Microbiology. 1st edition, Spectrum books Ltd. Ibadan, Nigeria, ISBN: 978-246-032-X
Federal Government of Nigeria â€˜FGNâ€™ 1997. Federal Government of Nigeria Annual Abstract of Statistics; FOS, Abuja, Nigeria. 477p.
Federal Ministry of Agriculture â€˜FMAâ€™ 1997. Federal Ministry of Agriculture: Annual Agricultural Statistics, PRSD, Abuja, Nigeria. 66p.
Ibrahim, S., Rahma, M. A. 2009. Isolation and Identification of Fungi associated with date Fruits (Phoenix dactylifera, Linn.) sold at Bayero University, Kano, Nigeria. Bayero Journal of Pure and Applied Sciences, 2(2): 127 â€“ 130.
Ita, E.O., Sado, E.K., Balogun, J.K., Pandogari, A., Ibitoye, B.A. 1984. â€œInventory survey of Nigeria Inland water and their fisheries resources.â€ A preliminary checklist on Inland water bodies in Nigeria with special reference to ponds, lakes, reservoir and major rivers, Kanji Lake Research Institute Technical report series 14, pp. 56-57.
James, G.C., Natalie. S. 2001. Microbiology. A Laboratory Manual (ed.). Pp. 211-223
Jimoh, W.A., Jabar, M.B., Adeleke, M.A., Bello, B.K. 2009. Bacterial isolates in the different gut regions of captured and cultured Clarias gariepinus in Abeokuta North Local Government. Nigerian Journal of Fisheries, 6 (1&2): 63-70.
Martinko, J.M and Madigan, M.T. 2006. Brock Biology of Microorganism. 11th Edition. ISBN 0-13-144329-1
Ogun State Agricultural Development Programme â€˜OGADEPâ€™ 1993. Ogun State Agricultural Development Programme. CAYS Publications, OGADEP, Abeokuta, Nigeria. Pp. 1-10.
Okonta, A.A., Ekelemu, J.K. 2005. A preliminary study of micro-organisms associated with fish spoilage in Asaba, Southern Nigeria. Proceedings of the 20th Annual Conference of the Fisheries Society of Nigeria (FISON), Port Harcourt, Nigeria. Pp. 557 â€“ 560.
Olaoye, O.J., Adekoya, B.B., Ezeri, G. N. O., Omoyinmi, G. A. K., Ayansanwo, T. O. 2007. Fish Hatchery Production Trends in Ogun State: 2001-2006. Journal of Field Aquatic Studies- Aquafield, 3: 29-40.
Olutiola, P.O., Famurewa, O., Sontag, H.G. 1991. An Introduction to general microbiology. A practical approach. 1stedition.Heidelberger Verlagsanstalt and Druckerei GmbH Heldelberg, Germany. ISBN: 3-89426-0.
Otwell, W.S., George, J.F. 2010. A HACCP Program for Raw, Cultured Penaeid Shrimp. Florida Sea Grant College Program. E/TP-1 P. 218
Pigott, G.M. 2009. Flavors and acceptance of formulated seafood products Food Reviews International Seafoods: Quality and Evaluation 6 :4
Shewan, J.M., Jones, N.R. 1957. Chemical changes occurring in cod muscles during chill storage and their possible use as objective indices of quality. Journal of Science, Food and Agriculture. 491-497
Taylor, D. S., Stone, M .B. 2008. â€œFood processing and preservationâ€ Redmond, Encarta 2009 [DVD]. Redmond, W.A: Microsoft corporation, 2008
Todar, K. 2008. The normal bacterial flora of humans. In: Textbook of Bacteriology. http://www.textbookofbacteriology.net/normalflora. html