AGRO-MORPHOLOGICAL VARIATION AND GENETIC POTENTIAL IN Vigna unguiculata subssp. unguiculata var. spontanea


  • O. A. ODUWAYE Department of Plant Breeding and Seed Technology
  • K. A. ADELEKAN Department of Plant Breeding and Seed Technology
  • O. J. ARIYO Department of Plant Breeding and Seed Technology
  • M. R. OLUBIYI National Centre for Biotechnology and Genetic Resources, Ibadan, Nigeria


cowpea, crop wild-relative (CWR), diversity, insect tolerance, multivariate analysis


Exploring the genetic potentials of wild relatives of crop varieties plays a critical role in broaden the narrow genetic base and introducing novel genetic diversity into the domesticated crop plants. Genetic diversity in 90 accessions of Vigna unguiculata subspp unguiculata var. spontanea and 3 cultivars of V. unguiculata subspp unguiculata var. unguiculata were investigated. Field trials were carried out at Abeokuta (2014 and 2015) and Ibadan (2014), Nigeria in a randomized complete block design with three replicates. Data collected on qualitative and quantitative traits varied among the accessions which indicated unique phenotypic features in the accessions. Early flowering accessions (NGB1140, NGB1083, NGB1136 and NGB1170) and accessions with low leaf defoliation (NGB1089, NGB1108, NGB1142, NGB1150, NGB1171, NGB1085 and NGB1177) among the cultivars were identified. Genetic diversity analysis revealed nineteen (19) homogenous groups among the accessions. Divergence among the groups was attributed more to seed yield (R2 = 0.90), number of pods/plant (R2 = 0.86) and days to flowering (R2 = 0.86). Promising genetic potential in the V. unguiculata subspp unguiculata var spontanea for desirable traits, and their effective use for further improvement of cultivated cowpea through hybridization programme were revealed.



Author Biographies

O. A. ODUWAYE, Department of Plant Breeding and Seed Technology

Federal University of Agriculture, Abeokuta, Nigeria

K. A. ADELEKAN, Department of Plant Breeding and Seed Technology

Federal University of Agriculture, Abeokuta, Nigeria

O. J. ARIYO, Department of Plant Breeding and Seed Technology

Federal University of Agriculture, Abeokuta, Nigeria


Ajeigbe, H.A., Singh, B.B., Emechebe, A.M. 2008. Field evaluation of improved cowpea lines for resistance to bacterial blight, virus and Striga under natural infestation in the West African Savannas. African Journal of Biotechnology 7: 3563-3568.

Alvey, S., Bagayoko, M., Neumann, G., Buerkert, A. 2001. Cereal/legume rotations affect chemical properties and biological activities in two West African soils. Plant Soil 231: 45–54.

Anele, U.Y., Sudekum, K.H., Arigbede, O.M., Luttgenau, H., Oni, A.O., Bolaji, O.J., Galyean, M.L. 2012. Chemical composition, rumen degradability and crude protein fractionation of some commercial and improved cowpea (Vigna unguiculata L. Walp) haulm varieties. Grass Forage Science 67(2): 210-218.

Asiwe, J.A.N. 2009. Insect mediated outcrossing and geneflow in cowpea (Vigna unguiculata (L.) Walp), Implication for seed production and provision of containment structures for genetically transformed cowpea. African Journal of Biotechnology 8: 226-230.

Bationo, A., Ntare, B.R. 2000. Rotation and nitrogen fertilizer effects on pearl millet, cowpea and groundnut yield and soil chemical properties in a sandy soil in the semi-arid tropics, West Africa. Journal of Agricultural Science 134: 277–284.

Boukar, O., Abberton, M., Oyatomi, O., Togola, A., Tripathi, L., Fatokun, C. 2020. Introgression breeding in cowpea [Vigna unguiculata (L.) Walp.]. Frontiers in Plant Science 11: 567425.

Breithaupt, H. 2008. Up to the challenge? Rising prices for food and oil could herald a renaissance of plant science. EMBO Reports 9: 832–834.

Brumlop, S., Reichenbecher, W., Tappeser, B., Finckh, M.R. 2013. What is the smartest way to breed plants and increase agrobiodiversity? Euphytica, 194: 53-66.

BT (International Board for Plant Genetic Resources. Descriptors for cowpea) 1983. Crop Genetic Resource Centre, Plant Production and Protection Division, Food and Agriculture Organization of the United Nations, Rome, Italy.

Coulibaly, P., Pasquet, R.S., Papa, R., Gepts, P. 2002. AFLP analysis of the phenetic organization and genetic diversity of Vigna unguiculata (L.) Walp. reveals extensive gene flow between wild and domesticated types. Theoretical and Applied Genetics 104: 358-266.

Da Silva, A.C., Santos, D.C., Junior, D.L.T. 2018. Cowpea, A Strategic Legume Species for Food Security and Health, IntechOpen, London, UK.

Ddamulira, G., Santos, C. 2015. Seed yield and protein content of Brazilian cowpea genotypes under diverse Mgandan environments. American Journal of Plant Science 6: 2074.

El-Niely Hanina, F.G. 2007. Effect of radiation processing on antinutrients, in-vitro protein digestibility and protein efficiency ratio bioassay of legume seeds. Radiation Physics and Chemistry 76: 1050–1057.

Fang, J.G., Chao, C.C.T., Roberts, P.A., Ehlers, J.D. 2007. Genetic diversity of cowpea [Vigna unguiculata (L.) Walp.] in four West African and USA breeding programs as determined by AFLP analysis. Genetic Resources and Crop Evolution, 54: 1197-1209.

Fatokun, C.A., Ng, Q. 2007. Outcrossing in cowpea. Journal of Food, Agriculture and Environment, 5: 334-338.

Fatokun, C.A., Tarawali, S.A., Sign, B.B., Korawa, P.M., Tumo, M. 2002. Challenges and opportunities for enhancing sustainable cowpea production. Proceedings of the 3rd World Cowpea Conference, September 4-8, 2000, International Institute of Tropical Agriculture IITA, Ibadan Nigeria pp. 214-338.

Feleke, Y., Pasquet, R.S., Gepts, P. 2006. Development of PCR-based chloroplast DNA markers to assess gene flow between wild and domesticated cowpea (Vigna unguiculata). Plant Systematic and Evolution 262(1/2): 75–87.

Gonçalves, A., Goufo, P., Barros, A., Dominguez-peris, R., Trindade, H., Rosa, E.A., Ferreira, L., Rodrigues, M. 2016. Cowpea (Vigna unguiculata L. Walp.), a renewed multipurpose crop for a more sustainable agri-food system, nutritional advantages and constraints. Journal of the Science of Food and Agriculture., 96(9): 2941-2951.

José, F., Cruz, R., Júnior De Almeida, H., Maria, D., Dos Santos, M. 2014. Growth, nutritional status and nitrogen metabolism in Vigna unguiculata (L.) Walp is affected by aluminum. Australian Journal of Crop Science 8: 1132– 1139.

Liyanage, R., Perera, O.S., Wethasinghe, P., Jayawardana, B.C., Vidanaarachchi, J.K., Sivaganesan, R. 2014. Nutritional properties and antioxidant content of commonly consumed cowpea cultivars in Sri Lanka. Journal of Food Legume 27(3): 215-217.

Mahalakshmi, V., Ng, Q., Lawson, M., Ortiz, R. 2007. Cowpea [Vigna unguiculata (L.) Walp.] core collection defined by geographical, agronomical and botanical descriptors. Plant Genetic Resources 5(3): 113–119.

Maxted, N., Kell, S.P. 2009. Establishment of a global network for the in-situ conservation of crop wild relatives, Status and needs. FAO Commission on Genetic Resources for Food and Agriculture, Rome, Italy.

McCouch, S., Baute, G.J., Bradeen, J., Bramel, P., Bretting, P.K., Buckler, E., Burke J.M., Charest, D., Cloutier, S., Cole, G., et al. 2013. Agriculture, Feeding the future. Nature 499: 23–24.

Meyer, R.S., Duval, A.E., Jensen, H.R. 2012. Patterns and processes in crop domestication, An historical review and quantitative analysis of 203 global food crops. New Phytologist 196: 29–48.

Olsen, K.M., Wendel, J.F. 2013. A bountiful harvest, Genomic insights into crop domestication phenotypes. Annual Review of Plant Biology 64: 47 – 70.

Pasquet, R.S. 1999. Genetic relationships among subspecies of Vigna unguiculata (L.) Walp. based on allozyme variation. Theoretical and Applied Genetics 98: 1104–1119.

Rahman, S.A., Ibrahim, U., Ajayi, F.A. 2008. Effect of defoliation at different growth stages on yield and profitability of cowpea (Vigna unguiculata (L.) Walp.). Electronic Journal of Environmental, Agricultural and Food Chemistry 7(9): 3248-3254.

Reis, C., Frederico, A. 2001. Genetic diversity in cowpea (Vigna unguiculata) using isozyme electrophoresis. Acta Horticulturae 546: 497–501.

SAS Institute, 2002. Statistical Analysis System for windows. Release 9.4 SAS Institute, Cary, North Carolina, USA.

Singh, S., Nag, S.K., Kundu, S.S., Maity, S.B. 2010. Relative intake, eating pattern, nutrient digestibility, nitrogen metabolism, fermentation pattern and growth performance of lambs fed organically and inorganically produced cowpea hay-barley grain diets. Tropical Grasslands 44: 55-61.

Ukpene, A.O., Imade, F.N. 2015. Amino acid profiles of seven cowpea varieties grown in Agbor. Nigerian Annals of Natural Sciences 15(1): 72-78.

Undal, V. S., Thakare, P. V., Chaudhari, U. S., Deshmukh, V. P., Gawande, P. A. 2011. Estimation of genetic diversity among wild Vigna species revealed by RAPD Markers. Annals of Biological Research 2(4): 348-354.

Warschefsky, E., Varma, P.R., Douglas, RC, Eric, J.B. 2014. Back to the wilds, tapping evolutionary adaptations for resilient crops through systematic hybridization with crop wild relatives. American Journal of Botany 101(10): 1791 – 1800.






Original Manuscript