DETERMINATION OF PROPERTIES OF A STRAIN OF TOBAMOVIRUS FROM MUCUNA PRURIENS

  • O. O. ODEDARA Department of Microbiology, Federal University of Agriculture, Abeokuta
  • A. C. ODEBODE Department of Botany, Faculty of Science, University of Ibadan, Ibadan
  • J. A. HUGHES International Rice Research Institute, Philliphines
Keywords: Incidence, Tobacco mosaic virus, necrosis, mottling, inactivation

Abstract

A virus-infected leaf of Mucuna pruriens was collected and tested for an incidence of a virus through biological properties using various plant host range inoculation tests, longevity-in-vitro, dilution-end-point, thermal-inactivation point and electron microscopy. Results of host range inoculation tests showed the following plant species to be susceptible to the virus; Nicotiana tabacum, N. rustica, N. benthamiana, N. glutinosa, N. occidentalis, N. Sylvesris (Speg and Comes) and others.

No plant in the family Convolvulacaee, Cucurbitaceae was found to be infected with the virus.  However Chenopodium quinoa and C. murale in the family Chenopodiaceae were infected with symptoms of mottling and necrotic lesions.  In the family Fabaceae, hybrid cowpea line TVu 76, IT84S – 2114 were infected with mosaic, leaf crinkling and severe leaf curl respectively. The virus had a dilution-end-point of 10-6-10-7, thermal-inactivation-point of 95°C in crude sap of TVu 76. The virus is of agricultural importance because of its incidence on seed coats of legumes which are freely exchanged between agricultural stakeholders.

 

 

 

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Published
2020-10-05
Section
Articles